Year: 2022
Type: preprint
Abstract: The animal reservoirs of sarbecoviruses represent a significant risk of emergent pandemics, as evidenced by the impact of SARS-CoV-2. Vaccines remain successful at limiting severe disease and death, however the continued emergence of SARS-CoV-2 variants, together with the potential for further coronavirus zoonosis, motivates the search for pan-coronavirus vaccines that induce broadly neutralizing antibodies. This necessitates a better understanding of the glycan shields of coronaviruses, which can occlude potential antibody epitopes on spike glycoproteins. Here, we compare the structure of several sarbecovirus glycan shields. Many N-linked glycan attachment sites are shared by all sarbecoviruses, and the processing state of certain sites is highly conserved. However, there are significant differences in the processing state at several glycan sites that surround the receptor binding domain. Our studies reveal similarities and differences in the glycosylation of sarbecoviruses and show how subtle changes in the protein sequence can have pronounced impacts on the glycan shield.
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: green
Awards: International AIDS Vaccine Initiative - to advance the CAVD-supported projects through the translational stage and into clinical evaluation, ultimately resulting in successful new HIV/AID..., G1597505487, G1926094088, The Scripps Research Institute - to establish a Center of Excellence for structure-guided immunogen design for global health-related problems, G3227429959, G4332762517, G479357336, G7876585708, G9000291502, Consortium for HIV/AIDS Vaccine Development
OpenAlex ID: W4293243287