Work

Exploring the Use of a Restoration Step to Detect Mosaic Chromosomal Alterations in Prostate Samples

Year: 2022

Type: conference-paper

Abstract: Genetic instability is a hallmark in tumor prevalence, resulting in aneuploidy and copy-neutral loss of heterozygosity (cn-LOH) events. 1 • The samples used in this pilot study were stored in formalin-fixed and paraffin-embedded (FFPE).Use of FFPE involves cross-linking, which causes DNA damage.2 • In addition, normal cell contamination and degradation in nucleic acid can make it difficult to identify mutations within a tumor sample due to dilution of the signal within the cells. 1 • The hapLOH method was developed to assist in the detection of copy-number events.When adding the restoration step, the DNA concentration is further reduced.

DOI: 10.52519/00017

Authors: Ayanna N. Woodberry, Justin W. Wong, Paul A. Scheet

Institutions: The University of Texas MD Anderson Cancer Center, Howard University

Language: English

Topic: Cancer Genomics and Diagnostics

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

OpenAlex ID: W4293280464

FWCI:

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