Insulin Prohormone Processing, Distribution, and Relation to Metabolism inAplysia californica
Year: 1999
Type: article
Abstract: The first Aplysia californica insulin gene is characterized and its proteolytic processing from prohormone to final peptides elucidated using a combination of biochemical and mass spectrometric methods. Aplysia insulin (AI) is one of the largest insulins found, with a molecular weight of 9146 Da, and an extended A chain compared with other invertebrate and vertebrate insulins. The AI prohormone produces a series of C peptides and also a unique N-terminally acetylated D peptide. AI-producing cells are restricted to the central region of the cerebral ganglia mostly within the F and C clusters, and AI is transported to neurohemal release sites located on the upper labial and anterior tentacular nerves. The expression of AI mRNA decreases when the animal is deprived of food, and injections of AI reduce hemolymph glucose levels, suggesting that the function of insulin-regulating metabolism has been conserved.
Source: Journal of Neuroscience
Authors: Philip D. Floyd, Lingjun Li, Stanislav S. Rubakhin, Jonathan V. Sweedler, Charles C. Horn, Irving Kupfermann, Vera Y. Alexeeva, Timothy A. Ellis, Nikolai C. Dembrow, Klaudiusz R. Weiss, Ferdinand S. Vilim
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: bronze
Awards: G1627089947, G1659482832, NETWORK, CELLULAR AND MOLECULAR DETERMINANTS OF BEHAVIOR, National Resource for Aplysia, BEHAVIORAL AND NEURAL ANALYSIS OF FEEDING IN APLYSIA, Neural Basis of Motivated Behaviors, Behavioral and Neurual Analysis of Learning in Aplysia, Neuropeptide Analysis using Multidimensional Separation and Detection Methodology, Isomerization in cell-to-cell signaling peptides: from discovery to function, G7265695668
OpenAlex ID: W1943620375