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Transcriptional profiling of mouse projection neurons with VECTORseq

Year: 2022
Type: article
Abstract: Existing techniques for transcriptional profiling of projection neurons could be applied to only one neuronal population per experiment. To increase throughput, we developed VECTORseq, which repurposes retrogradely infecting viruses to deliver multiplexable RNA barcodes, enabling projection anatomy to be read out in single-cell datasets. In this protocol, we describe the delivery of viral barcodes to mouse brain to label different projection neurons. We then detail single-cell or nuclei isolation for sequencing, followed by the analysis of single-cell sequencing data. For complete details on the use and execution of this protocol, please refer to Cheung et al. (2021).
DOI: 10.1016/j.xpro.2022.101625
Language: English
Open Access status: gold
Estimated APC paid: $1,500
Funders: Simons Foundation, Whitehall Foundation, Sandler Foundation, National Alliance for Research on Schizophrenia and Depression, James B. Pendleton Charitable Trust, E. Matilda Ziegler Foundation for the Blind, Simons Foundation Autism Research Initiative, National Institutes of Health, University of California, San Francisco, National Institute of Mental Health, National Institute of Allergy and Infectious Diseases, National Institute of Neurological Disorders and Stroke, National Center for Research Resources
Awards: High-resolution neural circuit dissection with controllers locally affecting synaptic partners (CLASP), Defining cell types that control action selection and execution, G4538197162, G463302875, G4805577867, BD FACSAria II Flow Cytometer, G4963622356, G5565511880, G705536867, G97119092
OpenAlex ID: W4293772125
FWCI:
Cites: 11
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Related to: 10