Work

Ultrastructural analysis of wild-type and RIM1α knockout active zones in a large cortical synapse

Year: 2022

Type: article

Abstract: Rab3A-interacting molecule (RIM) is crucial for fast Ca 2+ -triggered synaptic vesicle (SV) release in presynaptic active zones (AZs). We investigated hippocampal giant mossy fiber bouton (MFB) AZ architecture in 3D using electron tomography of rapid cryo-immobilized acute brain slices in RIM1α −/− and wild-type mice. In RIM1α −/− , AZs are larger with increased synaptic cleft widths and a 3-fold reduced number of tightly docked SVs (0–2 nm). The distance of tightly docked SVs to the AZ center is increased from 110 to 195 nm, and the width of their electron-dense material between outer SV membrane and AZ membrane is reduced. Furthermore, the SV pool in RIM1α −/− is more heterogeneous. Thus, RIM1α, besides its role in tight SV docking, is crucial for synaptic architecture and vesicle pool organization in MFBs.

Source: Cell Reports

DOI: 10.1016/j.celrep.2022.111382