Distinct local and brain-wide networks are activated by optogenetic stimulation of neurons specific to each layer of motor cortex
Year: 2022
Type: article
Abstract: Primary motor cortex (M1) consists of a stack of interconnected but distinct layers (L1-L6) which affect motor control through large-scale networks. However, the brain-wide functional influence of each layer is poorly understood. We sought to expand our knowledge of these layers' circuitry by combining Cre-driver mouse lines, optogenetics, fMRI, and electrophysiology. Neuronal activities initiated in Drd3 neurons (within L2/3) were mainly confined within M1, while stimulation of Scnn1a, Rbp4, and Ntsr1 neurons (within L4, L5, and L6, respectively) evoked distinct responses in M1 and motor-related subcortical regions, including striatum and motor thalamus. We also found that fMRI responses from targeted stimulations correlated with both local field potentials (LFPs) and spike changes. This study represents a step forward in our understanding of how different layers of primary motor cortex are embedded in brain-wide circuitry.
Source: NeuroImage
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,450
Awards: Foundations of MRI Corticography for mesoscale organization and neuronal circuitry, Innovating high-resolution novel imaging approaches to elucidate mechanisms of prion-like spreadingof neurodegenerative disease, Defining Cell Type Specific Contributions to fMRI Signals, G358049434, CRCNS: US-France-Israel Research Proposal: A personalized approach to brain stimulation, G7288184987, Animal 7T MRI Scanner for Imaging Neural Circuits, From Optogenetic Functional MRI to Mechanogenetic Functional Ultrasound
OpenAlex ID: W4296520646