Work

Protocol to utilize fresh uncultured human lung tumor cells for personalized functional diagnostics

Year: 2022

Type: article

Abstract: Drug sensitivity data acquired from solid tumor-derived cultures are often unsuitable for personalized treatment guidance due to the lengthy turnaround time. Here, we present a protocol for determining ex vivo drug sensitivities using fresh uncultured human lung tumor-derived EpCAM + epithelial cells (FUTCs). We describe steps for drug testing in FUTCs to identify tumor cell-selective single or combination therapy in 72 h of sample processing. The FUTC-based approach can also be used to predict in vivo resistance to known targeted therapies. For complete details on the use and execution of this protocol, please refer to Talwelkar et al. (2021) .

Source: STAR Protocols

DOI: 10.1016/j.xpro.2022.101720

Authors: Sarang S. Talwelkar, Iris A.K. Lähdeniemi, Mikko I. Mäyränpää, Annabrita Hemmes, Nora Linnavirta, Jari Räsänen, Aija Knuuttila, Krister Wennerberg, Emmy W. Verschuren

Institutions: University of Helsinki, University of Turku, Institute for Molecular Medicine Finland, HiLIFE – Elämäntieteiden Instituutti, Helsinki University Hospital, University of Copenhagen

Language: English

Topic: Lung Cancer Treatments and Mutations

Subfield: Pulmonary and Respiratory Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,500

Funders: Ida Montinin Säätiö, Helsingin Yliopisto, Suomen Lääketieteen Säätiö, Medicinska Understödsföreningen Liv och Hälsa, European Commission, Academy of Finland, Novo Nordisk, Väinö ja Laina Kiven Säätiö, Novo Nordisk Fonden, Biocenter Finland, Horizon 2020 Framework Programme, Helsinki Institute of Life Science, Helsingin Yliopisto, Horizon 2020, H2020 Marie Skłodowska-Curie Actions

Awards: G155838707, G3589578894, G399059165, G434337789, Innovative strategies for cancer prevention with focus on sex hormone signaling and chronic inflammation, Development of new antimicrobial nanostructured durable coatings for fuel tanks, Innovative strategies for cancer prevention with focus on sex hormone signaling and chronic inflammation, G7044228676, G7940337353

OpenAlex ID: W4297314868

FWCI:

Cites: 7

Cited by:

Related to: 10