Work

SSI 2013 Abstract Submission Innate Immunity

Year: 2013

Type: article

Abstract: Background: Collectins and ficolins are important in the clearance of endogenous and exogenous danger materials.A new human collectin-11 was recently identified in serum with low concentration in complex with mannose-binding lectin (MBL)/ficolin-associated serine proteases, and found to recognize carbohydrate residues present on various microorganisms.Objectives: We hypothesized that collectin-11 could be a novel initiation molecule in the lectin complement pathway in addition to the MBL and the ficolins.Methods: The association of MASP-1, -2, -3 and MAP-1 with collectin-11 was analyzed by the ELISA platform and by flow cytometry using Candida albicans as collectin-11 binding matrix.The potential involvement of collectin-11 in activation of the lectin complement pathway was evaluated by addition of exogenous recombinant MASP-2 and purified human C4 to collectin-11 anchored on Candida albicans using flow cytometry.Moreover, we investigated the depositions of C4, C3 and the terminal complement complex (TCC) in the presence of collectin-11 on Candida albicans by using MBL-defect serum as complement source.Results: We found that collectin-11 could associate with all the known MASPs (MASP-1, -2, -3) as well as the lectin pathway regulator MAP-1, and revealed that collectin-11:MASP-2 complex formation on Candida albicans could mediate C4 deposition.Furthermore, collectin-11 anchored on Candida albicans induced depositions of C4, C3 and formation of TCC in the presence of MBL-defect serum.Conclusions: These results define collectin-11 as the fifth recognition molecule in the lectin complement pathway in addition to MBL, ficolin-1, ficolin-2 and ficolin-3.

Source: Scandinavian Journal of Immunology

DOI: 10.1111/sji.12036