Fluorimetry Study of N‐(1‐Pyrenyl)iodoacetamide‐Labelled F‐Actin: Local Structural Change of Actin Protomer both on Polymerization and on Binding of Heavy Meromyosin
Year: 1981
Type: article
Abstract: A fluorescent reagent, N-(1-pyrenyl)iodoacetamide, was conjugated to rabbit skeletal muscle actin at the site of the most reactive sulfhydryl group, and fluorescence characteristics (excitation and emission spectra, quantum yields, lifetimes) of the conjugate were investigated. Associated with polymerization of labelled G-actin, the fluorescence intensity at 407 nm, after excitation at 365 nm, was enhanced by a factor of about 25. It was reduced to about 25% on the binding of heavy meromyosin (or subfragment 1). The results suggest that binding of heavy meromyosin to the protomer of F-actin alters the local structure of the protomer towards a G-actin-like one.
Source: European Journal of Biochemistry
Authors: Tsutomu KOUYAMA, Koshin MIHASHI
Institution: Nagoya University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W4297917728