Work

Light Regulation of Protein Dimerization and Kinase Activity in Living Cells Using Photocaged Rapamycin and Engineered FKBP

Year: 2020

Type: article

Abstract: We developed a new system for light-induced protein dimerization in living cells using a novel photocaged analog of rapamycin (pRap) together with an engineered rapamycin binding domain (iFKBP). Using focal adhesion kinase as a target, we demonstrated successful light-mediated regulation of protein interaction and localization in living cells. Modification of this approach enabled light-triggered activation of a protein kinase and initiation of kinase-induced phenotypic changes in vivo.

Source: Carolina Digital Repository (University of North Carolina at Chapel Hill)

DOI: 10.17615/5pz5-7c82

Authors: Young, Douglas D., Karginov, Andrei V., Zou, Yan, Dokholyan, Nikolay V., Kota, Pradeep, Hahn, Klaus M., Shirvanyants, David, Deiters, Alexander

Language: English

Topic: Photochromic and Fluorescence Chemistry

Subfield: Materials Chemistry

Field: Materials Science

Domain: Physical Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: Switchable Systems for Spatio-Temporal Control of Gene Expression in Zebrafish, G9000006921, G9000084312, G9000112765, G9000152630

OpenAlex ID: W4298060468

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