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REGENERATION AND Agrobacierlum - MEDIATED TRANS- . FORMATION STUDIES IN FIG; Ficus carica L.

Year: 2006
Type: article
Abstract: A system for regeneration and Agrobacferium mediated transformation of the common figFr'cus caries L. of the cultivar Sultani was developed. Different combinations of BA and NAA or 2,4-D and kinetin of callus formation from leaf segment explants. and the best % formation of callus was found to be 2.0 mg/L 2,4-D and 0.2 mgfL kinetin. Callus produced somatic embryogenesis which differentiated to plantlets on media containing TDZ in combination with NAA or 2ip.The best concentrations which produce somatic embryogenesis and differentiated to plantlets on media were containingSO mgfl 2ip and 7 mg/L TDZ with 0.25 mg/L NAA, which gave the regeneration efficiencies 83 and 79 of respectively.The callus failed to induce organogenesis on media containing BA and kinetin. The best concentrations of growth regulators to produce shoots formation by direct somatic embryogenesis with leaf explants were on media containing 2 mg/L TDZ and 4 mg/L 2ip, which gave the regeneration efficiencies (89%).The best results of shoots forming roots was reached the highest value 95% by using MS medium supplemented with 1.0 mg/ L IBA. Leaf explants of in w'tro propagated plants were co-cultivation with Agrcbacferium cell culture containing the binary plasmid plSV267B which harbor the gus-intron and bar genes, the results showed that the highest transformation efficiency was obtained when explants incubated for 30 min. with bacterial suspension (17.5%). The transgenic nature of regenerated plants was confirmed by histochemical GUS, leaf painting assay and PCB analysis. Keywords: Fig (Ficus canoe); Transformation; Callus; Regeneration; GUS; bar, Agrobacterium fumefaciens
DOI: 10.21608/jpp.2006.236335
Language: English
Subfield: Plant Science
Open Access status: diamond
OpenAlex ID: W4299150842
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