Work

Global analysis of genomic instability caused by DNA replication stress in Saccharomyces cerevisiae

Year: 2016

Type: article

Abstract: phase, presumably as a consequence of broken replication forks. We observed a high rate of chromosome loss, likely reflecting a reduced capacity of the low-polymerase strains to repair double-stranded DNA breaks (DSBs). We also observed a high frequency of deletion events within tandemly repeated genes such as the ribosomal RNA genes. By whole-genome sequencing, we found that low levels of DNA polymerase δ elevated mutation rates, both single-base mutations and small insertions/deletions. Finally, we showed that cells with low levels of DNA polymerase δ tended to accumulate small promoter mutations that increased the expression of this polymerase. These deletions conferred a selective growth advantage to cells, demonstrating that DRS can be one factor driving phenotypic evolution.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.1618129113

Authors: Dao-Qiong Zheng, Ke Zhang, Xue-Chang Wu, Piotr A. Mieczkowski, Thomas D. Petes

Institutions: Duke University, Zhejiang University, University of North Carolina at Chapel Hill

Language: English

Topic: DNA Repair Mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Natural Science Foundation of China, National Institutes of Health, National Institute of General Medical Sciences, National Institute of Environmental Health Sciences