Work

Nanogrid rolling circle DNA sequencing

Year: 2023

Type: article

Abstract: The present invention relates to methods for sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size, including synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sectional diameter greater than the diameter of a specific region, immobilizing the concatemer to the specific region to make an immobilized concatemer, and sequencing the immobilized concatemer.

Source: OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information)

Author: Church, George M.; Porreca, Gregory J.; Shendure, Jay; Rosenbaum, Abraham Meir

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W4299851693

FWCI:

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Related to: 10