Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies
Year: 2020
Type: article
Abstract: Researchers rely largely on antibodies to measure the abundance, activity, and localization of a protein, information that provides critical insight into both normal and pathological cellular functions. However, antibodies are not always reliable or universally valid for the methods in which they are used; in particular, the reliability of commercial antibodies against RAS is highly variable. Waters et al . rigorously assessed 22 commercially available RAS antibodies for their utility to detect the distinct RAS isoforms in various cell types and for their use in specific analytical methods. Their findings show how reliably one can interpret the data acquired from each reagent.
DOI: 10.17615/penz-qv09
Authors: Mccormick, Frank, Philips, Mark R., Tessier-Cloutier, Basile, Fer, Nicole, Huntsman, David G., Waters, Andrew M., Houghton, Peter J., Strathern, Leslie A., Hartley, James L., Ozkan-Dagliyan, Irem, Cox, Adrienne D., Gillette, William K., Der, Channing J., Hobbs, G. Aaron, Vaseva, Angelina V., Bagni, Rachel, Whiteley, Gordon R.
Institution: University of North Carolina at Chapel Hill
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4300667644