Work

Dosage-dependent proteome response of Shewanella oneidensis MR-1 to chromate insult

Year: 2006

Type: article

Abstract: Shewanella oneidensis MR-1 is a gram-negative, facultatively anaerobic bacterium originally isolated from a freshwater lake. S. oneidensis MR-1 has the ability to reduce toxic metal ions [e.g., Cr(VI) and U(VI)] found in industrial and governmental waste sites. Cells were grown and exposed to three different metal concentrations in order to probe the dosage response of S. oneidensis MR-1 to Cr(VI) in the form of chromate. Protein fractions were digested with trypsin and analyzed with a multidimensional HPLC-NanoESIMS/MS protocol. The goal of this work is to identify protein components of pathways/mechanisms responsible for both detoxification and reduction of chromate.

Source: OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information)

Authors: Melissa Thompson, Nathan C. VerBerkmoes, Karuna Chourey, Steven D. Brown, Robert L. Hettich, Dorothea K. Thompson

Language: English

Topic: Chromium effects and bioremediation

Subfield: Health, Toxicology and Mutagenesis

Field: Environmental Science

Domain: Physical Sciences

Open Access status: green

OpenAlex ID: W4301015230

FWCI:

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