Work

Invasive cleavage of nucleic acids

Year: 2023

Type: article

Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

Source: OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information)

Author: Prudent, James R [Madison, WI]; Hall, Jeff G [Madison, WI]; Lyamichev, Victor I [Madison, WI]; Brow, Mary Ann D [Madison, WI]; Dahlberg, James E [Madison, WI]

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W4302063845

FWCI:

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Related to: 10