Work

Advances in nanopore direct RNA sequencing

Year: 2022

Type: article

Abstract: Nanopore direct RNA sequencing (DRS) reads continuous native RNA strands. Early adopters have used this technology to document nucleotide modifications and 3′ polyadenosine tails on RNA strands without added chemistry steps. Individual strands ranging in length from 70 to 26,000 nucleotides have been sequenced. In our opinion, broader acceptance of nanopore DRS by molecular biologists and cell biologists will be accelerated by higher basecall accuracy and lower RNA input requirements.

Source: Nature Methods

DOI: 10.1038/s41592-022-01633-w

Authors: Miten Jain, Robin Abu-Shumays, Hugh E. Olsen, Mark Akeson

Institutions: Northeastern University, University of California, Santa Cruz

Language: English

Topic: Nanopore and Nanochannel Transport Studies

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $11,390

Funders: National Institutes of Health, National Human Genome Research Institute