Gut microbiota and plasma metabolites associated with bone mineral density in women with or at risk of HIV infection
Year: 2022
Type: article
Study design: Observational Study
Abstract: OBJECTIVE: To evaluate gut microbiota (GMB) alterations and metabolite profile perturbations associated with bone mineral density (BMD) in the context of HIV infection. DESIGN: Cross-sectional studies of 58 women with chronic HIV infection receiving antiretroviral therapy and 33 women without HIV infection. METHODS: We examined associations of GMB and metabolites with BMD among 91 women. BMD was measured by dual-energy X-ray absorptiometry (DXA), and T -scores of lumbar spine or total hip less than -1 defined low BMD. GMB was measured by 16S rRNA V4 region sequencing on fecal samples, and plasma metabolites were measured by liquid chromatography-tandem mass spectrometry. Associations of GMB with plasma metabolites were assessed in a larger sample (418 women; 280 HIV+ and 138 HIV-). RESULTS: Relative abundances of five predominant bacterial genera ( Dorea , Megasphaera , unclassified Lachnospiraceae, Ruminococcus , and Mitsuokella ) were higher in women with low BMD compared with those with normal BMD (all linear discriminant analysis (LDA) scores >2.0). A distinct plasma metabolite profile was identified in women with low BMD, featuring lower levels of several metabolites belonging to amino acids, carnitines, caffeine, fatty acids, pyridines, and retinoids, compared with those with normal BMD. BMD-associated bacterial genera, especially Megasphaera , were inversely associated with several BMD-related metabolites (e.g. 4-pyridoxic acid, C4 carnitine, creatinine, and dimethylglycine). The inverse association of Megasphaera with dimethylglycine was more pronounced in women with HIV infection compared with those without HIV infection ( P for interaction = 0.016). CONCLUSION: Among women with and at risk of HIV infection, we identified altered GMB and plasma metabolite profiles associated with low BMD.
Source: AIDS
Institutions: Albert Einstein College of Medicine, Fudan University, State Key Laboratory of Genetic Engineering, Columbia University Irving Medical Center, Johns Hopkins University, Hektoen Institute, Rush University Medical Center, SUNY Downstate Health Sciences University, Fred Hutch Cancer Center, Harvard University
Language: English
Topic: Gut microbiota and health
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funders: National Institutes of Health, National Heart, Lung, and Blood Institute, National Cancer Institute, National Institute of Allergy and Infectious Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Center for Advancing Translational Sciences, National Institute on Minority Health and Health Disparities, Eunice Kennedy Shriver National Institute of Child Health and Human Development
Awards: Liver Pathobiology and Gene Therapy Research Core Center, Evaluation of HIV-associated Cardiac Dysfunction in Women, Emory WIHS CRS - Longitudinal Cohort, Inflammatory and Immune Mechanisms of Atherosclerosis in HIV-Infected Women, The Connie Wofsy Women's HIV Study, Brooklyn Women's Interagency HIV Study (WIHS) V, Miami Womens Interagency HIV Study WIHS, Advanced Glycation Endproducts and Cardio-Renal Disease, WIHS Data Management and Analysis Center (WDMAC), Human Gut Microbiome and Incident Diabetes Risk in U.S. Populations, Southern California Women's Interagency HIV Study Consortium, Epidemiology of the gut microbiome, prediabetes and diabetes in Latinos, Mechanisms of Atherosclerosis and CVD in HIV+ Women, Chicago WIHS Consortium V (CWC), Washington Women's Interagency HIV/AIDS Study, Role of innate immunity in HIV related vascular disease: biomarkers & mechanisms, Integrated analysis of CVD risk in HIV: gut microbiota, immune function and metabolites, HIV Infection, Metabolites and Subclinical Atherosclerosis, Clinical and Translational Science Institute, Atlanta Clinical and Translational Science Institute (ACTSI) Renewal, Metabolomics signatures underlying diet, lifestyle and gut microbiota for diabetes, HIV and the menopausal transition: effects on musculoskeletal health, University of North Carolina Womens Interagency HIV Study UNC WIHS, Columbia Collaborative HIV/AIDS Clinical Trials Unit, Diabetes Research and Training Center, Women's Interagency HIV Study (WIHS-V), UAB-MISS WIHS Cohort, ERC Einstein-Rockefeller-CUNY Center for AIDS research, Cancer Center Support Grant, MACS/WIHS Combined Cohort Study: Cook County Clinical Research Site (CC_CRS), Mentoring Research in Non-infectious Complications of HIV, The University of North Carolina Center for AIDS Research, Columbia Collaborative HIV/AIDS Clinical Trials Unit, MACS/WIHS Combined Cohort Study: Brooklyn Clinical Research Site (Bklyn CRS), Clinical Research Sites for the MACS/WIHS Combined Cohort Study (MACS/WIHS-CCS)
OpenAlex ID: W4303423730