Work

The expanding CRISPR toolbox for natural product discovery and engineering in filamentous fungi

Year: 2022

Type: article

Abstract: CRISPR/Cas technologies has opened up avenues for the manipulation of biosynthetic gene clusters across diverse filamentous fungi. This review discusses the innovative and diverse strategies for fungal natural product discovery and engineering made possible by CRISPR/Cas-based tools. We also provide a guide into multiple angles of CRISPR/Cas experiment design, and discuss current gaps in genetic tool development for filamentous fungi and the promising opportunities for natural product research.

Source: Natural Product Reports

DOI: 10.1039/d2np00055e

Authors: Clara Woodcraft, Yit-Heng Chooi, Indra Roux

Institution: The University of Western Australia

Language: English

Topic: Microbial Natural Products and Biosynthesis

Subfield: Pharmacology

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: University of Western Australia, Australian Research Council

Awards: The hidden secondary metabolite biosynthetic potential of fungi, CRISPR-based pathway activation for bioactive molecule discovery in fungi