Compact simultaneous label-free autofluorescence multi-harmonic (SLAM) microscopy for user-friendly photodamage-monitored imaging
Year: 2022
Type: preprint
Abstract: Label-free nonlinear optical microscopy has become a powerful tool for biomedical research. However, the possible photodamage risk hinder further clinical applications. To reduce these adverse effects, we constructed a new platform of simultaneous label-free autofluorescence multiharmonic (SLAM) microscopy, featuring 5-channel multimodal imaging, inline photodamage monitoring, and pulse repetition-rate tuning. By the use of a birefringent photonic crystal fiber for spectral broadening (rather than supercontinuum generation) and a prism compressor for pulse pre-chirping, this system allows users to independently adjust pulse width, repetition rate, and energy, which is useful for optimizing imaging condition towards no/minimal photodamage. Also, it demonstrates label-free multichannel imaging at one excitation pulse per image pixel and thus paves the way for improving the imaging speed by a faster optical scanner.
Source: arXiv (Cornell University)
Authors: Wang, Geng, Boppart, Stephen A., Tu, Haohua
Institution: University of Illinois Urbana-Champaign
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: green
Award: G1753018743
OpenAlex ID: W4307415657