Year: 2022
Type: article
Abstract: Many applications seek to measure a sample’s absorption coefficient spectrum to retrieve the chemical makeup. Many real-world samples are optically turbid, causing scattering confounds which many commercial spectrometers cannot address. Using diffusion theory and considering absorption and reduced scattering coefficients on the order of 0.01 mm−1 and 1mm−1, respectively, we develop a method which utilizes frequency-domain to measure absolute optical properties of turbid samples in a standard cuvette (45 mm×10 mm×10 mm). Inspired by the self-calibrating method, which removes instrumental confounds, the method uses measurements of the diffuse complex transmittance at two sets of two different source-detector distances. We find: this works best for highly scattering samples (reduced scattering coefficient above 1 mm−1); higher relative error in the absorption coefficient compared to the reduced scattering coefficient; accuracy is tied to knowledge of the sample’s index of refraction. Noise simulations with 0.1% amplitude and 0.1°=1.7 mrad phase uncertainty find errors in absorption and reduced scattering coefficients of 4% and 1%, respectively. We expect that higher error in the absorption coefficient can be alleviated with highly scattering samples and that boundary condition confounds may be suppressed by designing a cuvette with high index of refraction. Further work will investigate implementation and reproducibility.
Source: Applied Sciences
DOI: 10.3390/app122110903
Authors: Giles Blaney, Angelo Sassaroli, Sergio Fantini
Institution: Tufts University
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,408
OpenAlex ID: W4307539260