Work

Transposon-containing DNA cloning vector and uses thereof

Year: 2023

Type: article

Abstract: The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed.

Source: OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information)

Author: Berg, Claire M [W. Willington, CT]; Berg, Douglas E [St. Louis, MO]; Wang, Gan [Storrs, CT]

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W4307580255

FWCI:

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Related to: 10