Work

3-dimensional imaging at nanometer resolutions

Year: 2023

Type: article

Abstract: An apparatus and method for enabling precise, 3-dimensional, photoactivation localization microscopy (PALM) using selective, two-photon activation of fluorophores in a single z-slice of a sample in cooperation with time-gated imaging for reducing the background radiation from other image planes to levels suitable for single-molecule detection and spatial location, are described.

Source: OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information)

Author: Werner, James H [Los Alamos, NM]; Goodwin, Peter M [Los Alamos, NM]; Shreve, Andrew P [Santa Fe, NM]

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W4307618087

FWCI:

Cites:

Cited by:

Related to: 10