Year: 2022
Type: article
Abstract: We have determined the susceptibility of T4 DNA (166 kilobase pairs, kbp) to fragmentation under steady shear in a cone-and-plate rheometer. After shearing for at least 30 min at a shear rate of 6000s−1, corresponding to a Reynolds number of O(103) and a Weissenberg number of O(103), 97.9±1.3% of the sample is broken into a polydisperse mixture with a number-averaged molecular weight of 62.6±3.2 kbp and a polydispersity index of 1.29±0.03, as measured by pulsed-field gel electrophoresis (with a 95% confidence interval). The molecular weight distributions observed here from a shear flow are similar to those produced by a (dominantly extensional) sink flow of DNA and are qualitatively different than the midpoint scission observed in simple extensional flow. Given the inability of shear flow to produce a sharp coil–stretch transition, the data presented here support a model where polymers can be fragmented in flow without complete extension. These results further indicate that DNA fragmentation by shear is unlikely to be a significant issue in microfluidic devices, and anomalous molecular weight observations in experiments are due to DNA processing prior to observation in the device.
Source: Biomicrofluidics
DOI: 10.1063/5.0109361
Institution: University of Minnesota
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W4307975857