Year: 2022
Type: article
Abstract: Vasopressin has traditionally been thought to be produced by the neurohypophyseal system and then released into the circulation where it regulates water homeostasis. The questions of whether vasopressin could be produced outside of the brain and if the kidney could be a source of vasopressin are raised by the syndrome of inappropriate antidiuretic hormone secretion (vasopressin). We found that mouse and human kidneys expressed vasopressin mRNA. Using an antibody that detects preprovasopressin, we found that immunoreactive preprovasopressin protein was found in mouse and human kidneys. Moreover, we found that murine collecting duct cells made biologically active vasopressin, which increased in response to NaCl-mediated hypertonicity, and that water restriction increased the abundance of kidney-derived vasopressin mRNA and protein expression in mouse kidneys. Thus, we provide evidence of biologically active production of kidney-derived vasopressin in kidney tubular epithelial cells.
Source: JCI Insight
Authors: Juan Pablo Arroyo, Andrew S. Terker, Yvonne Zuchowski, Jason A. Watts, Fabian Bock, Cameron Meyer, Wentian Luo, Meghan Kapp, Edward R. Gould, Adam X. Miranda, Joshua Carty, Ming Jiang, Roberto Vanacore, Elizabeth Hammock, Matthew H. Wilson, Roy Zent, Mingzhi Zhang, Gautam Bhave, Raymond C. Harris
Institutions: Vanderbilt University Medical Center, National Institutes of Health, National Institute of Environmental Health Sciences, University Hospitals of Cleveland, Case Western Reserve University, Vanderbilt University, Florida State University, United States Department of Veterans Affairs, VA Tennessee Valley Healthcare System
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $4,100
Funders: U.S. Department of Veterans Affairs, Robert Wood Johnson Foundation, American Heart Association, American Society of Nephrology, Vanderbilt University, ASN Foundation for Kidney Research, Vanderbilt University Medical Center, National Institutes of Health, National Heart, Lung, and Blood Institute, National Institute of Mental Health, National Institute on Drug Abuse, National Human Genome Research Institute, NIH Office of the Director, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Neurological Disorders and Stroke, National Center for Advancing Translational Sciences, Common Fund
Awards: G1187429744, G1280513408, G1281419676, Mechanisms of EGFR Activation and Signaling in Kidney Disease, G1433450062, G2006259475, The role of lysyl oxidase like-2 in chronic kidney disease, Renal Biology and Disease Training Program, G2532530556, G3141307551, G336524319, Vanderbilt O'Brien Kidney Center, G3961473130, G4028326642, G4252985639, G4410787187, G4513176822, G4702718958, The Role of Hypobromous Acid in Renal Disease, Role of Eicosanoids in Renal Function, G526617753, G5580019600, Prune Belly Syndrome: Mechanisms of Filamin A Mutations, G5820162814, G5853943267, G6112956297, G6297262231, G659731888, G7488990925, G8245716232, G8999469958, G9000081609, G9000130502, Vanderbilt Stimulating Access to Research in Residency (V-StARR), Vanderbilt Institute for Clinical and Translational Research (VICTR), The role of the innate immune cell cyclooxygenase/prostaglandin system in blood pressureregulation, Cancer Center Support Grant, Innovative therapeutic approaches to treat chronic kidney disease, The Laminin Receptors in Kidney Disease, Role of the innate immune system in acute kidney injury, Medical Scientist Training Program
OpenAlex ID: W4308052485