Designing a quality assurance process for quality control of nuclear magnetic resonance metabolomics studies of human blood
Year: 2022
Type: article
Abstract: High-resolution magic angle spinning (HRMAS) nuclear magnetic resonance (NMR)-based metabolomics has demonstrated its utility in studies of biofluids for various diseases. HRMAS NMR spectroscopy is uniquely well suited for analyzing human blood samples because of the small quantity of samples and minimal preparation required. To develop this methodology into standardized clinical protocols, establishment of the method's quality assurance (QA) and evaluations of its quality control (QC) are critical. This study aims to assess the QA/QC measured from human blood specimens in the form of serum and plasma through within-subject and between-subject comparisons, as well as stability and consistency comparisons over several freezing-thawing cycles of sample storage conditions, and most importantly, the agreement of pooled control samples against individual samples.
Source: NMR in Biomedicine
DOI: 10.1002/nbm.4868
Institutions: Harvard University, Massachusetts General Hospital
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Awards: General Anesthesia and Alzheimer's Disease Neuropathogenesis, Upgrade of Console for 600 MHz MR Spectrometer, Identification of prostate cancer aggressiveness using hyperpolarized polyamine metabolic rates, Characterizing Prostate Cancer By ex vivo MRS Signatures, Novel metabolomic contrast probes for human lung cancer characterization, Metabolomic characterization of normal aging and Alzheimer's disease under anesthesia- surgery stimuli, Massachusetts Alzheimer's Disease Research Center (MADRC)
OpenAlex ID: W4308181467