Exosomes loaded with miR-665 inhibit the progression of osteosarcoma in vivo and in vitro.
Year: 2022
Type: article
Abstract: OBJECTIVE: Osteosarcoma (OS) is the most common primary malignant bone tumor and has a poor prognosis. Recent research has suggested that miR-665 affects the progression of OS. Moreover, an exosome delivery system presents better targeting effects, higher permeability, and lower immunogenicity than other nano-delivery systems do. The purpose of this study is to explore whether an exosome loaded with the miR-665 delivery system can inhibit OS development. METHODS: , respectively. Cell migration and invasion were measured using scratch and transwell assays. Engineered exosomes were prepared using electroporation. H&E staining was employed to observe necrotic cells and the function of heart, liver, spleen, lung and kidney. The expression of proteins was estimated by immunoblot analysis. RESULTS: . Furthermore, we verified that the exosome delivery system exhibited good safety and target efficiency. CONCLUSION: in a safe manner.
Source: PubMed
Institution: Affiliated Hospital of Nantong University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4309413002