Year: 2022
Type: article
Abstract: SignificanceCerebral metabolic rate of oxygen (CMRO2) consumption is a key physiological variable that characterizes brain metabolism in a steady state and during functional activation.AimWe aim to develop a minimally invasive optical technique for real-time measurement of CMRO2 concurrently with cerebral blood flow (CBF).ApproachWe used a pair of macromolecular phosphorescent probes with nonoverlapping optical spectra, which were localized in the intra- and extravascular compartments of the brain tissue, thus providing a readout of oxygen gradients between these two compartments. In parallel, we measured CBF using laser speckle contrast imaging.ResultsThe method enables computation and tracking of CMRO2 during functional activation with high temporal resolution (∼7 Hz). In contrast to other approaches, our assessment of CMRO2 does not require measurements of CBF or hemoglobin oxygen saturation.ConclusionsThe independent records of intravascular and extravascular partial pressures of oxygen, CBF, and CMRO2 provide information about the physiological events that accompany neuronal activation, creating opportunities for dynamic quantification of brain metabolism.
Source: Neurophotonics
Institutions: University of Pennsylvania, University of South Florida
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,675
OpenAlex ID: W4310112815