MASM inhibits cancer stem cell-like characteristics of EpCAM+ cells via AKT/GSK3β/β-catenin signaling.
Year: 2022
Type: article
Abstract: OBJECTIVES: HCC cells. METHODS: HCC cells was determined by the sphere-formation and cell counting kit 8 assays. After these cell populations were exposed to increasing concentrations of MASM, sphere formation, cell proliferation, apoptosis, resistance to chemotherapy and colony formation were evaluated, respectively. Moreover, the stemness-associated gene expression and underlying mechanisms were evaluated by quantitative real-time polymerase chain reaction and sphere-forming assay. RESULTS: HCC cells and up-regulated mature hepatocyte-related genes. Moreover, MASM suppressed the formation and reduced the size of not only primary spheroids but also subsequent spheroids. Additionally, our results showed that MASM inhibited the AKT/GSK3β/β-catenin signaling pathway. CONCLUSION: cells and may be considered as a novel drug candidate in HCC therapy.
Source: PubMed
Authors: Keyan Sun, Huaxing Shen, Shipeng He, Ying Liu
Institution: Shanghai University
Language: English
Topic: Cancer Cells and Metastasis
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4311290657