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Chemically defined generation of human definitive hematopoietic stem and progenitor cells

Year: 2022
Type: article
Abstract: Here, we present a protocol to efficiently direct human pluripotent stem cells (hPSCs) into hematopoietic stem and progenitor cells (HSPCs) under a chemically defined, albumin-free system. We describe the induction of aorta-gonad-mesonephros-like hematopoiesis from hPSCs into SOX17+ hemogenic endothelium and then into CD34+CD45+ HSPCs via application of Wnt activator and TGFβ inhibitor, respectively. The generated HSPCs, characterized by flow cytometry and colony-forming unit assay, express definitive hematopoiesis markers and exhibit multilineage differentiation potential and the capacity to expand. For complete details on the use and execution of this protocol, please refer to Chang et al. (2022a, 2022b).1,2
DOI: 10.1016/j.xpro.2022.101953
Authors: Juhyung Jung, Yun Chang, Gyuhyung Jin, Qing Deng, Xiaojun Lance Lian, Xiaoping Bao
Language: English
Open Access status: gold
Estimated APC paid: $1,500
Funders: National Science Foundation, Purdue University, National Institutes of Health, Davidson School of Chemical Engineering, Purdue University, National Cancer Institute, National Institute of General Medical Sciences, Division of Chemical, Bioengineering, Environmental, and Transport Systems
Awards: G109959128, Cancer Center Support Grant, CAREER: Engineering ex vivo Human Cardiogenesis with Optogenetics, G6325092012, CAREER: Enhancing Stem Cell Differentiation with CRISPR Activators via Non-integrating Methods, Engineer Biomimetic Microfluidic Models to Investigate and Reprogram Tumor Associated Neutrophils for Cancer Therapy
OpenAlex ID: W4311825038
FWCI: 0.276
Cites: 7
Cited by: 4
Related to: 10