Work

Genetically encoded multimeric tags for intracellular protein localisation in cryo-EM

Year: 2022

Type: preprint

Abstract: Cryo-electron tomography is a powerful label-free tool for visualizing biomolecules in their native cellular context at molecular resolution. However, the precise localisation of biomolecules of interest in the tomographic volumes is challenging. Here, we present a tagging strategy for intracellular protein localisation based on genetically encoded multimeric particles (GEMs). We show the applicability of drug-controlled GEM labelling of endogenous proteins in cryo-electron tomography and cryo-correlative fluorescence imaging in human cells.

Source: bioRxiv (Cold Spring Harbor Laboratory)

DOI: 10.1101/2022.12.10.519870

Authors: Herman KH Fung, Yuki Hayashi, Veijo T Salo, Anastasiia Babenko, Ievgeniia Zagoriy, Andreas Brunner, Jan Ellenberg, Christoph W Müller, Sara Cuylen-Haering, Julia Mahamid

Institutions: European Molecular Biology Laboratory, Heidelberg University

Language: English

Topic: Advanced Electron Microscopy Techniques and Applications

Subfield: Structural Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: European Molecular Biology Laboratory, Boehringer Ingelheim Fonds, Deutsche Forschungsgemeinschaft, Biomedicum Helsinki-säätiö, Boehringer Ingelheim Stiftung, H2020 Marie Skłodowska-Curie Actions

Awards: Koordinationsfonds, G4328676618, G4376339242, SPP 2191: Molekulare Mechanismen funktioneller Phasenseparation, EMBL Interdisciplinary, International and Intersectorial Postdocs

OpenAlex ID: W4312097612

Cites: 58

Cited by: 4

Related to: 10