Bioremediation of Spent Engine Oil Contaminated Soil by Using Fungus, Penicillium sp.
Year: 2016
Type: article
Abstract: This study investigated the ability ofPenicillium sp.to bio-remediate spent engine oil contaminated soil bothin vitroandin vivo. In thein vitroassay, mycelium of a seven day old culture ofPenicillium sp. grown on Sabouraud Dextrose Agar (SDA) was punched out using a 0.5mm Cork borer and inoculated on the centre of Petri dishes containing the spent and unspent engine oil and incubated for seven days and daily reading of the mycelia growth obtained using a metre rule. For thein vivoassay, soil received 0 (control), 20/180, 40/360, 60/540, 80/720 and 100ml/900mm concentrations/treatments (inoculation with mycelium ofPenicillium sp.). Seeds ofTelfeira occidentaliswas sown on the soil and assessed for growth performance (plant height, leaf area (using a metre rule) and leaf count (number of leaves) for 7, 14, 21 and 28 Days after Planting (DAP). Results of thein vitroassay showed a significant increase (p<0.05) in the growth diameter ofPenicillium sp.relative to control. Results of thein vivoassay showed that spent engine oil had no significant effect (p<0.05) on the growth performance ofT. occidentalisat 7, 14, 21 and 28 DAP and on fresh and dry weight (g) 28 DAP relative to control. After 28 days of plant growth, the added spent engine oil was no longer detected. The plant began producing pods 61 DAP. This study showed thatPenicillium sp. can biodegrade hydrocarbons present in spent engine oil and as such is a good tool for bioremediation.
DOI: 10.56431/p-q41iwn
Institution: University of Calabar
Language: English
Subfield: Pollution
Field: Environmental Science
Domain: Physical Sciences
Open Access status: hybrid
OpenAlex ID: W2532088752