Galectin-3 synergizes with CD47 to Suppress Phagocytosis and T cell immunity in Peritoneal Metastases of Gastric Adenocarcinoma
Year: 2022
Type: conference-paper
Study design: Observational Study
Abstract: A. Bulk RNA-seq analysis on Peritoneal metastasis (PC) specimen using a curated panel of immune checkpoint and "don't eat me" gene. B. Bulk RNA-seq analysis on Stage IV of TCGA STAD (n=41) cohort using a notable panel of immune checkpoint and "don't eat me" gene. C. Expression profile of "don't eat me" genes CD47, SIRPA, CD274, PDCD1, LILRB1 and LILRB2 were shown in dot-plot with normalized expression levels of indicated genes from scRNAseq data (n=20) . D. CD47 and PD-L1 were detected on 6 representative PC ascites samples, as examined by CyTOF using antibodies against notable cell type markers, and CD47 and PD-L1. E. CD47 expression (right) on EpCAM + tumor cells detected on PC samples between long-term survivor (>12 month) and shortterm survivor (<12 month) (n=42), as assessed by flow cytometry . F. Normalized expression levels of CD47 and SIRPA in tumor cluster and myeloid cluster between long-term survivors and shortterm survivors was shown as violin plots G. The overall survival of CD47 in complete responders (CR) GAC patients from TCGA dataset Z-Score -0.5
DOI: 10.52519/00054
Institution: The University of Texas MD Anderson Cancer Center
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W4312508365