Work

Characterization of T/B cell antigen specific-engineered tumors for studying T cell and GC B cell function in a murine model of NSCLC

Year: 2022

Type: conference-paper

Abstract: Methods HypothesisWe hypothesized that HELLO and NINJA antigenexpressing NSCLC cells will be useful models to study T/B cell interaction within the tumor microenvironment. Results Conclusions• Tumor growth was significantly decreased in the 344SQ-NINJA model, compared to 344SQ-HELLO and 344SQ-LentiT models.• Infiltration of antigen-specific CD4, CD8 T cells and cytotoxic CD8 T cells was increased in NINJA tumors compared to Lenti-T tumors.• NINJA tumors presented higher infiltration of CD4 TFH cells, which may suggest a role of this subpopulation in the antitumor response.• HELLO tumors presented higher infiltration of Tregs, which may play a role in the reduced antitumor effect of HELLO vs NINJA antigens.• The higher amount of activated Tet+ CD8+ T cells in dLNs of HELLO vs that of NINJA and LentiT tumor-bearing mice suggests potential distinct tumor antigenspecific immune responses in these models.

DOI: 10.52519/00080