The effect of temperature on the critical electric field for electroporation: A single cell electroporation device study
Year: 2017
Type: article
Abstract: Electroporation is the permeabilization of the cell membrane with brief, high electric fields. It is a technology of importance in bioengineering and medicine. An experimental study was performed to determine the effect of temperature on the electroporation parameters in individual cells. For this purpose, we designed a microelectromechanical device that maintains a controlled temperature and detects the threshold of electroporation by incorporating an individual cell into an electronic circuit. A series of experiments were performed with Madin-Darby Canine Kidney (MDCK) cells. We have found that the electrical field corresponding to the onset of electroporation, known as the critical electric field (EC), is a strong function of temperature. It increases with decreasing temperature. The critical electric field for electroporation at 5o C is as much as two times that needed to electroporate the cells at 37o C.
Authors: DIAZ-RIVERA RUBENE, RUBINSKY BORIS
Institution: University of California, Berkeley
Language: English
Subfield: Biotechnology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W4313119695