Work

Surface Expression and Functional Competence of CD3-Independent TCR ζ-Chains in Immature Thymocytes

Year: 1999

Type: article

Abstract: In recombinase-deficient (RAG-2−/−) mice, double-negative thymocytes can be stimulated to proliferate and differentiate by anti-CD3 Abs. CD3 molecules are expressed on the surface of these cells in association with calnexin. In this study, we show that ζ-chains can be recovered as phosphorylated proteins in association with phosphorylated ZAP-70 from anti-CD3-stimulated RAG-2−/− thymocytes, even though they are not demonstrably associated with the CD3/calnexin complex. The lack of a physical association of ζ dimers with the CD3 complex in RAG-2−/− thymocytes and also in a pre-TCR-expressing cell line, as well as the efficient association of ζ dimers with ZAP-70 in the RAG-2−/− thymocytes, suggest that these ζ-chain dimers could contribute to pre-TCR signaling. This idea is supported by the finding that in RAG-2−/− ζ-deficient thymocytes, ZAP-70 and p120cbl were only weakly phosphorylated.

Source: The Journal of Immunology

DOI: 10.4049/jimmunol.162.5.2589

Authors: Fabio Grassi, Eliane Barbier, Simona Porcellini, Harald von Boehmer, Pierre-André Cazenave

Institutions: Centre National de la Recherche Scientifique, Institut Pasteur, University of Milan, Université Pierre-et-Marie-Curie, Inserm

Language: English

Topic: T-cell and B-cell Immunology

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: Association pour la Recherche sur le Cancer