Work

An optogenetic tool to inhibit RhoA in Drosophila embryos

Year: 2023

Type: article

Abstract: We describe a protocol for optogenetic inhibition of the small GTPase Rho1 (RhoA) in Drosophila embryos, which allows rapid and spatially confined inactivation of Rho1 and Rho1-mediated actomyosin contractility. We provide step-by-step instruction for optogenetic manipulations of Drosophila embryos using confocal and multiphoton imaging systems. This tool is useful for determining the site- and stage-specific function of Rho1 in Drosophila embryos and for studying the immediate tissue response to acute elimination of cellular contractility. For complete details on the use and execution of this protocol, please refer to Guo et al. (2022).1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2022.101972

Authors: Hanqing Guo, Michael Swan, Bing He

Institutions: Dartmouth College, Princeton University

Language: English

Topic: Photoreceptor and optogenetics research

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,500

Funders: American Cancer Society, Dartmouth College, National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases