Work

A protocol for collection and infusion of cerebrospinal fluid in mice

Year: 2023

Type: article

Abstract: Here, we provide a step-by-step protocol for the collection and intracerebroventricular infusion of cerebrospinal fluid (CSF) in mice. We describe steps to withdraw CSF quickly and abundantly while avoiding blood contamination. Using the Lynch coil technique, we gain functional insights into the collected CSF by slowly infusing minimal amounts of CSF directly to the lateral ventricles of aged mice. This protocol is versatile and can be used to infuse drugs, antibodies, or scarce biological compounds. For complete details on the use and execution of this protocol, please refer to Iram et al. (2022).1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2022.102015

Authors: Achint Kaur, Steven Shuken, Andrew Chris Yang, Tal Iram

Institutions: Stanford Medicine, Stanford University, University of California, San Francisco

Language: English

Topic: Cerebrospinal fluid and hydrocephalus

Subfield: Clinical Neurology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,500