Monitoring cell fate in 3D organotypic human squamous epithelial cultures
Year: 2023
Type: article
Abstract: Here, we provide a protocol to model the effects of changes to a small number of cells, such as those arising from a mutation or a virus infection, in stratified epithelia. We describe steps for diluting engineered human keratinocytes into a larger population of unmodified cells and using these cells to grow three-dimensional organotypic cultures. We detail steps to observe effects that are not apparent in homogenous organotypic epithelial cultures by visualizing the localization of modified keratinocytes in epithelial layers. For complete details on the use and execution of this protocol, please refer to Hatterschide et al. (2022).1
Source: STAR Protocols
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,500
Funders: American Cancer Society, University of Pennsylvania, National Institutes of Health, Penn Skin Biology and Diseases Resource-based Center, University of Pennsylvania, National Institute of Allergy and Infectious Diseases, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Dental and Craniofacial Research
Awards: G1283609449, G1813920882, Training in Virology, Human Papillomavirus Manipulation of Epithelial Differentiation, G5741372456, Investigating the mechanism by which PTPN14 degradation by HPV E7 represses epithelial differentiation, G8200358429, Penn Skin Biology and Diseases Resource-based Center, G960013086
OpenAlex ID: W4319301400