Targeting MYH9 represses USP14-mediated NAP1L1 deubiquitination and cell proliferation in glioma
Year: 2023
Type: article
Abstract: Myosin heavy chain 9 (MYH9) plays an important role in a number of diseases. Nevertheless, the function of MYH9 in glioma is unclear. The present research aimed to investigate the role of MYH9 in glioma and determine whether MYH9 is involved in the temozolomide chemoresistance of glioma cells. Our results showed that MYH9 increased the proliferation and temozolomide resistance of glioma cells. The mechanistic experiments showed that the binding of MYH9 to NAP1L1, a potential promoter of tumor proliferation, inhibited the ubiquitination and degradation of NAP1L1 by recruiting USP14. Upregulation of NAP1L1 increased its binding with c-Myc and activated c-Myc, which induced the expression of CCND1/CDK4, promoting glioma cell temozolomide resistance and proliferation. Additionally, we found that MYH9 upregulation was strongly related to patient survival and is therefore a negative factor for patients with glioma. Altogether, our results show that MYH9 plays a role in glioma progression by regulating NAP1L1 deubiquitination. Thus, targeting MYH9 is a potential therapeutic strategy for the clinical treatment of glioma in the future.
Source: Cancer Cell International
Institutions: Central South University, TCM-Intigrated Cancer Center of Southern Medical University, Southern Medical University, Youjiang Medical College for Nationalities, Affiliated Hospital of Youjiang Medical University for Nationalities, Hunan Normal University, Hunan Provincial People's Hospital, First Affiliated Hospital Zhejiang University
Language: English
Subfield: Genetics
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,890
Awards: G2667284573, G2875548408, G5884771680
OpenAlex ID: W4387141230