Year: 2023
Type: article
Abstract: SignificanceBased on acoustic detection of optical absorption, photoacoustic tomography (PAT) allows functional and molecular imaging beyond the optical diffusion limit with high spatial resolution. However, multispectral functional and molecular PAT is often limited by decreased spectroscopic accuracy and reduced detection sensitivity in deep tissues, mainly due to wavelength-dependent optical attenuation and inaccurate acoustic inversion.AimPrevious work has demonstrated that reversible color-shifting can drastically improve the detection sensitivity of PAT by suppressing nonswitching background signals. We aim to develop a new color switching-based PAT method using reversibly switchable thermochromics (ReST).ApproachWe developed a family of ReST with excellent water dispersion, biostability, and temperature-controlled color changes by surface modification of commercial thermochromic microcapsules with the hydrophilic polysaccharide alginate.ResultsThe optical absorbance of the ReST was switched on and off repeatedly by modulating the surrounding temperature, allowing differential photoacoustic detection that effectively suppressed the nonswitching background signal and substantially improved image contrast and detection sensitivity. We demonstrate reversible thermal-switching imaging of ReST in vitro and in vivo using three PAT modes at different length scales.ConclusionsReST-enabled PAT is a promising technology for high-sensitivity deep tissue imaging of molecular activity in temperature-related biomedical applications, such as cancer thermotherapy.
Source: Journal of Biomedical Optics
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $1,675
Awards: G168841704, CAREER: Mapping deep brain functions with super-resolution photoacoustic imaging, Photoacoustic-imaging-guided intravascular sonothrombolysis, G2107305235, G2284106056, G2427575068, G2618681103, G2922183890, High-Throughput Volumetric Photoacoustic Imaging of Living Vascularized Organoids, G4259659471, Transcranial Photoacoustic Imaging Using a Virtual Array, Head-mounted Photoacoustic Imaging of Deep-brain Neural Activities in Freely Behaving Animals, G5586775963, G6671297155, G6693263171, 3D Functional Photoacoustic Imaging of Human Brain with a Stretchable Ultrasound Matrix Array, G8126308421, G8433557135, High-resolution High-speed Photoacoustic and Ultrasound Imaging of SmallVessel Functions in Ischemic Stroke, High-throughput Imaging-integrated Vascular Model for Understanding Thromboembolism and Therapeutics Screening
OpenAlex ID: W4321078072