Nanowarming and ice-free cryopreservation of large sized, intact porcine articular cartilage
Year: 2023
Type: article
Abstract: Successful organ or tissue long-term preservation would revolutionize biomedicine. Cartilage cryopreservation enables prolonged shelf life of articular cartilage, posing the prospect to broaden the implementation of promising osteochondral allograft (OCA) transplantation for cartilage repair. However, cryopreserved large sized cartilage cannot be successfully warmed with the conventional convection warming approach due to its limited warming rate, blocking its clinical potential. Here, we develope a nanowarming and ice-free cryopreservation method for large sized, intact articular cartilage preservation. Our method achieves a heating rate of 76.8 °C min-1, over one order of magnitude higher than convection warming (4.8 °C min-1). Using systematic cell and tissue level tests, we demonstrate the superior performance of our method in preserving large cartilage. A depth-dependent preservation manner is also observed and recapitulated through magnetic resonance imaging and computational modeling. Finally, we show that the delivery of nanoparticles to the OCA bone side could be a feasible direction for further optimization of our method. This study pioneers the application of nanowarming and ice-free cryopreservation for large articular cartilage and provides valuable insights for future technique development, paving the way for clinical applications of cryopreserved cartilage.
Source: Communications Biology
Institutions: Clemson University, Medical University of South Carolina
Language: English
Subfield: Rheumatology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,690
Funders: U.S. Department of Health and Human Services, University of South Carolina, National Institutes of Health, Hollings Cancer Center, Medical University of South Carolina, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Dental and Craniofacial Research
Awards: G1410469566, Ice-free vitrification and nanowarming of large osteochondral grafts for transplantation, G204654204, G3549828030, Confocal/Multiphoton Microscope Upgrade, G4314960743, Tissue structure and mechanical function relationships of the human temporomandibular lateral capsule-ligament: Investigation of sexual and racial dimorphisms, South Carolina COBRE in Oxidants, Redox Balance and Stress Signaling, G8204968129, SC COBRE for TranslationalResearch Improving MusculoskeletalHealth (SC-TRIMH), T-COHR: Training in Craniofacial and Oral Health Research, Integrating craniofacial morphology, oral function, temporomandibular biomechanics and mechanobiology to determine sex-specific TMJ pathophysiology in humans, Medical University of South Carolina - Cancer Center Support Grant
OpenAlex ID: W4321791315