Work

Combining experimental evolution with next-generation sequencing: a powerful tool to study adaptation from standing genetic variation

Year: 2014

Type: article

Abstract: Evolve and resequence (E&R) is a new approach to investigate the genomic responses to selection during experimental evolution. By using whole genome sequencing of pools of individuals (Pool-Seq), this method can identify selected variants in controlled and replicable experimental settings. Reviewing the current state of the field, we show that E&R can be powerful enough to identify causative genes and possibly even single-nucleotide polymorphisms. We also discuss how the experimental design and the complexity of the trait could result in a large number of false positive candidates. We suggest experimental and analytical strategies to maximize the power of E&R to uncover the genotype-phenotype link and serve as an important research tool for a broad range of evolutionary questions.

Source: Heredity

DOI: 10.1038/hdy.2014.86

Authors: C Schlötterer, R Kofler, E Versace, R Tobler, S U Franssen

Institutions: University of Veterinary Medicine Vienna, University of Trento

Language: English

Topic: Evolution and Genetic Dynamics

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $2,690

Funder: Austrian Science Fund