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Giving Gold Wings: Ultrabright and Fragmentation Free Mass Spectrometry Reporters for Barcoding, Bioconjugation Monitoring, and Data Storage

Year: 2023
Type: article
Abstract: The widespread application of laser desorption/ionization mass spectrometry (LDI-MS) highlights the need for a bright and multiplexable labeling platform. While ligand-capped Au nanoparticles (AuNPs) have emerged as a promising LDI-MS contrast agent, the predominant thiol ligands suffer from low ion yields and extensive fragmentation. In this work, we develop a N-heterocyclic carbene (NHC) ligand platform that enhances AuNP LDI-MS performance. NHC scaffolds are tuned to generate barcoded AuNPs which, when benchmarked against thiol-AuNPs, are bright mass tags and form unfragmented ions in high yield. To illustrate the transformative potential of NHC ligands, the mass tags were employed in three orthogonal applications: monitoring a bioconjugation reaction, performing multiplexed imaging, and storing and reading encoded information. These results demonstrate that NHC-nanoparticle systems are an ideal platform for LDI-MS and greatly broaden the scope of nanoparticle contrast agents.
DOI: 10.1002/anie.202219182
Authors: Nathaniel L. Dominique, Isabel M. Jensen, Gurkiran Kaur, Chandler Q. Kotseos, William C. Boggess, David M. Jenkins, Jon P. Camden
Language: English
Subfield: Spectroscopy
Field: Chemistry
Open Access status: bronze
Awards: Collaborative Research: Characterization and Optimization of N-Heterocyclic Carbene Functionalized Nanoparticle Systems, G2351931133, Collaborative Research: Characterization and Optimization of N-Heterocyclic Carbene Functionalized Nanoparticle Systems, G476511176, G8657272577, G938035178
OpenAlex ID: W4322617482
FWCI: 1.105
Cites: 77
Cited by: 12
Related to: 10