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KSHV Viral Protein Kinase Interacts with USP9X to Modulate the Viral Lifecycle

Year: 2023
Type: article
Abstract: Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiological agent of Kaposi sarcoma (KS), the plasmablastic form of multicentric Castleman's disease, and primary effusion lymphoma. In sub-Saharan Africa, KS is the most common HIV-related malignancy. KSHV encodes a viral protein kinase (vPK) that aids viral replication. To elucidate the interactions of vPK with cellular proteins in KSHV-infected cells, we used an affinity purification approach and identified host protein ubiquitin-specific peptidase 9X-linked (USP9X) as a potential interactor of vPK. Depletion of USP9X inhibits both viral reactivation and the production of infectious virions. Overall, our data suggest a proviral role for USP9X.
DOI: 10.1128/jvi.01763-22
Authors: Danielle L. Chappell, Praneet K. Sandhu, Jason P. Wong, Aadra P. Bhatt, Xiaoxi Liu, Sara J. Buhrlage, Brenda R. S. Temple, M. Ben Major, Blossom Damania
Language: English
Subfield: Oncology
Field: Medicine
Open Access status: bronze
Awards: G5668739698, G5983290771, Mechanisms of KSHV Transmission, G6936146552, Role of KSHV Viral Proteins in Signaling and Pathogenesis, G9000157298, Targeted Therapies for HIV-Associated Kaposi Sarcoma and Lymphoma, Viral Oncogenesis, Latency, and Replication, Cancer Center Support Grant
OpenAlex ID: W4323311653
FWCI: 0.971
Cites: 51
Cited by: 8
Related to: 10