Assessment of Tobacco Fields for the Prevalence of Verticillium dahliae in District Swabi, Pakistan
Year: 2023
Type: article
Abstract: Field and laboratory investigations of tobacco plants were carried out to analyze the distribution of Verticillium wilt caused by V. dahliae. V. dahliae is isolated from field Z (Tarkhai, Yarhussain region of Swabi). Species of Fusarium and Actinomycetes were detected in field B (Shaheedan, Yarhussain region of Swabi), while no V. dahliae was isolated from these fields. V. dahliae was identified based on its colony and conidia morphology. The colony was white, having oval spores and septate mycelium with phialides. The isolate also produced irregularly elongated dark brown to black color microsclerotia of various sizes. The spores count for V. dahliae isolate was 3.61 × 108 conidia/ml. The average colony diameter of V. dahliae after 9 days of incubation on PDA and CM media was 6.55 cm and 5.5 cm, respectively. The biology of Verticillium spp. in tobacco plants and soil in Swabi has not been studied. For the first time, this study reported the presence of V. dahliae in the tobacco fields of Swabi, KPK Pakistan. Detection and isolation of V. dahliae from field soil are necessary for studying the ecology and virulence and creating deterrent control strategies for Verticillium wilt diseases.
Authors: Tasiq Ahmad, Said Akram, Shah Fahad, Muhammad Ilyas, Mukhtar Alam, Hazrat Hussain, Latifur Rehman
Institutions: University of Swabi, Kohsar University Murree
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $250
OpenAlex ID: W4323320949