Work

Analysis of human GPER expression in normal tissues and select cancers using immunohistochemistry

Year: 2023

Type: preprint

Abstract: GPER (G protein-coupled estrogen receptor) has been reported to play roles in several areas of physiology including cancer, metabolic disorders, and cardiovascular disease. However, the understanding of where this receptor is expressed in human tissue is limited due to limited available tools and methodologies that can reliably detect GPER protein. Recently, a highly specific monoclonal antibody against GPER (20H15L21) was developed and is suitable for immunohistochemistry. Using this antibody, we show that GPER protein expression varies markedly between normal human tissue, and also among cancer tissue. As GPER is an emerging therapeutic target for cancer and other diseases, this new understanding of GPER distribution will likely be helpful in design and interpretation of ongoing and future GPER research.

Source: bioRxiv (Cold Spring Harbor Laboratory)

DOI: 10.1101/2023.03.09.531931

Authors: Christopher A. Natale, John T. Seykora, Todd W Ridky

Institution: University of Pennsylvania

Language: English

Topic: Estrogen and related hormone effects

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute, National Institute of Environmental Health Sciences, National Institute of Arthritis and Musculoskeletal and Skin Diseases