The cellular and KSHV A-to-I RNA editome in primary effusion lymphoma and its role in the viral lifecycle
Year: 2023
Type: article
Abstract: Adenosine-to-inosine RNA editing is a major contributor to transcriptome diversity in animals with far-reaching biological consequences. Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiological agent of several human malignancies including primary effusion lymphoma (PEL). The extent of RNA editing within the KSHV transcriptome is unclear as is its contribution to the viral lifecycle. Here, we leverage a combination of biochemical and genomic approaches to determine the RNA editing landscape in host- and KSHV transcriptomes during both latent and lytic replication in PEL. Analysis of RNA editomes reveals it is dynamic, with increased editing upon reactivation and the potential to deregulate pathways critical for latency and tumorigenesis. In addition, we identify conserved RNA editing events within a viral microRNA and discover their role in miRNA biogenesis as well as viral infection. Together, these results describe the editome of PEL cells as well as a critical role for A-to-I editing in the KSHV lifecycle.
Source: Nature Communications
Language: English
Topic: RNA regulation and disease
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $6,290
Funders: U.S. Department of Health and Human Services, American Cancer Society, Pew Charitable Trusts, Vanderbilt University, Cleveland Clinic, Vanderbilt University Medical Center, National Institutes of Health, Lerner Research Institute, Cleveland Clinic, National Cancer Institute, National Institute of Allergy and Infectious Diseases
OpenAlex ID: W4324128690