High-speed imaging of cilia beat frequency using phase-resolved spectrally encoded interferometric microscopy
Year: 2023
Type: conference-paper
Abstract: Mucociliary clearance is vital for preventing any foreign substances from entering the upper airway that can later develop into acute and/or chronic respiratory diseases. Therefore, it is essential to further advance our understanding of the mucociliary functions. Our lab has been able to make key developments in imaging cilia, specifically measuring cilia beat frequency, with phase-resolved Doppler optical coherence tomography. In this system, we have further developed the system by incorporating phase-resolved spectrally encoded interferometric microscopy (SIEM) system with an FDML laser with MHz sweep rate to image cilia with higher accuracy and to minimize motion artifacts. In addition, we have designed a compact handheld probe system with a GRIN lens for easier in vivo imaging. The development of this system will allow us to further investigate cilia dynamics and ultimately utilize the system for clinical applications.
DOI: 10.1117/12.2651305
Authors: Do A Kim, Zhikai Zhu, Danny Chou, Katelyn Dilley, Theodore V. Nguyen, Edward Kuan, Brian Wong, Zhongping Chen
Institution: University of California, Irvine
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W4324134016