Work

Modeling nonlinear optical microscopy in scattering media, part I. Propagation from lens to focal volume: tutorial

Year: 2023

Type: article

Abstract: The development and application of nonlinear optical (NLO) microscopy methods in biomedical research have experienced rapid growth over the past three decades. Despite the compelling power of these methods, optical scattering limits their practical use in biological tissues. This tutorial offers a model-based approach illustrating how analytical methods from classical electromagnetism can be employed to comprehensively model NLO microscopy in scattering media. In Part I, we quantitatively model focused beam propagation in non-scattering and scattering media from the lens to focal volume. In Part II, we model signal generation, radiation, and far-field detection. Moreover, we detail modeling approaches for major optical microscopy modalities including classical fluorescence, multi-photon fluorescence, second harmonic generation, and coherent anti-Stokes Raman microscopy.

Source: Journal of the Optical Society of America A

DOI: 10.1364/josaa.478712

Authors: Janaka C. Ranasinghesagara, Eric O. Potma, Vasan Venugopalan

Institutions: University of California, Irvine, Beckman Laser Institute and Medical Clinic

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Science Foundation, National Institute of General Medical Sciences, Division of Chemical, Bioengineering, Environmental, and Transport Systems

Awards: Collaborative Research: Leveraging light scattering in tissues for improved biomedical imaging, Adaptive optics modeling in nonlinear microscopy to improve deep tissue imaging, G8445342530